【京辰生科】公司介紹
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商品介紹
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【訂製抗體】客製化抗體服務 單株多株抗體訂製服務 antibody抗體訂製3
【京辰生科】公司介紹 【京辰生科】台灣經銷/代理 商品介紹 【客製化抗體服務】 訂製抗體 【訂製抗體】客製化抗體服務 單株多株抗體訂製服務 antibody抗體訂製
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【訂製抗體】客製化抗體服務 單株多株抗體訂製服務 antibody抗體訂製

 【訂製抗體】客製化抗體服務  單株多株抗體訂製服務 Antibody抗體訂製 (Customized Antibody Service)

Polyclonal      
項目編號 服務項目 服務工作 提供客戶成品 時間
P1 Polyclonal package 1
(From protein to antisera)
protein check
immunization and sera collection
ELISA identification
1 ml pre-immune serum
>50 ml antisera
ELISA result (>1:10000 )
Western blot report
2-3 moths
P2 Polyclonal package 2
(From protein to protein A/G purified antibodies)
protein check
immunization and sera collection
20 ml antisera purification
ELISA identification
1 ml pre-immune serum
> 40 ml antisera
>20 mg purified antibodies
ELISA result (>1:10000 )
Western blot report
2-3 moths
P3 Polyclonal package 3
(From protein to affinity purified antibodies)
protein check
immunization and sera collection
20 ml antisera purification
ELISA identification
1 ml pre-immune serum
> 40 ml antisera
purified antibodies
ELISA result
Western blot report
3 months
P4 Polyclonal package 4
(From peptide design to antisera)
peptide design and synthesis
peptide conjugation
immunization and sera collection
ELISA identification
4 mg peptide + 5 mg peptide-KLH
1 ml pre-immune serum
>50 ml antisera
ELISA result (>1:4000 )
Dot blot report
2-3 moths
P5 Polyclonal package 5
(From peptide design to protein A/G purified antibodies)
peptide design and synthesis
peptide conjugation
immunization and sera collection
20 ml antisera purification
ELISA identification
4 mg peptide + 5 mg peptide-KLH
1 ml pre-immune serum
> 40 ml antisera
 purified antibodies
ELISA result
Dot blot report
3 months
Monoclonal    
項目編號 服務項目 服務工作 提供客戶成品 時間
M1 Monoclonal package 1
(From protein to hybridomas)
protein check
immunization and sera collection
fusion and hybridoma development
ELISA identification
Subcloning and cell cryopreservation
20 ul pre-immune sera
positive hybridomas (2 clones)
ELISA result
Western Blot report
5-6 months
M2 Monoclonal package 2
(From protein to ascites)
protein check
immunization and sera collection
fusion and hybridoma development
ELISA identification
Subcloning and cell cryopreservation
ascites production
20 ul pre-immune sera
positive hybridomas (2 clones)
3 ml ascites (1 clone)
ELISA result
Western Blot report
5-6 months
M3 Monoclonal package 3
(From protein to ascites purification)
protein check
immunization and sera collection
fusion and hybridoma development
ELISA identification
Subcloning and cell cryopreservation
ascites production and purification (<5 ml="" td="">555
20 ul pre-immune sera
positive hybridomas (2 clones)
ascites (1 clone )
5 mg protein A/G purified antibodies
ELISA result
Western Blot report
6-7 months
M4 Monoclonal package 4
(From peptide to ascites)
peptide design and synthesis
peptide conjugation
immunization and sera collection
fusion and hybridoma development
ELISA identification
Subcloning and cell cryopreservation
ascites production
4 mg peptide + 5 mg peptide-KLH
20 ul pre-immune sera
positive hybridomas (2 clones)
3 ml ascites (1 clone)
ELISA result
Dot blot report
5-6 months
M5 Monoclonal package 5
(From peptide to ascites purification )
peptide design and synthesis
peptide conjugation
immunization and sera collection
fusion and hybridoma development
ELISA identification
Subcloning and cell cryopreservation
ascites production purification (<5 ml="" td="">555
4 mg peptide + 5mg peptide-KLH
20 ul pre-immune sera
positive hybridomas (2 clones)
ascites (1 clone)
5 mg protein A/G purified antibodies
ELISA result
Dot blot report
6-7 months

乙醯化抗體

專案 時間 抗原材料  
乙醯化多株抗體 3個月  乙醯化peptide
非乙醯化peptide
出貨標準>0.2mg已純化磷酸化抗體+剩下血清

>Pre-Serum 兩隻兔子各1mL
血清 當作 negative control 可以確認兔子裡面沒有會跟客戶抗原反應的抗體  以防止偽陽性反應的結果
>免疫3-4次時提供Pre-Test Serum供客戶實驗挑選其中一隻兔子繼續進行後續免疫與血清純化
多株抗體免疫失敗材料費     多株抗體免疫失敗定義:若免疫之抗原效價皆符合出貨規格,但客戶實際檢體無法運用。則不出貨但需收取材料費。
乙醯化單株抗體 5-6個月  乙醯化peptide
非乙醯化peptide
出貨標準: 2株細胞株 (>106 Cells)+ 3ml 腹水 (1株)

出貨5株細胞培養液 (5ml)供客戶測試,最後可挑選2株細胞株,最後可挑選1株陽性株進行腹水生產。陽性株定義:ELISA吸收值扣除背景值後>0.5吸收值。
單株抗體免疫失敗材料費     單株抗體免疫失敗定義:若免疫之抗原效價(peptide 或protien)皆符合出貨規格,但客戶實際檢體無法運用。則不出貨但需收取材料費。
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