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1.00045.0010 MUP Selective Supplement (Bifido Selective Supplement A FD250 ) TOS MUP3
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1.00045.0010 MUP Selective Supplement (Bifido Selective Supplement A FD250 ) TOS MUP

(MUP) Bifido Selective Supplement A 原廠網站 Click here

乳酸菌相關培養基
Lactobacilli MRS agar/Lactobacilli MRS broth
Transgalactosylated oligosaccharides-mupirocin medium (TOS-MUP)
Tryptose-sulfite cycloserine (TSC) agar


(MUP) Bifido Selective Supplement A FD250    FD250-5VL(5支/盒) Lithium mupirocin

A selective supplement recommended for the selective isolation of Bifidobacterium species by colony count technique from milk products. Composition Per vial sufficient for 500 ml medium *Ingredients Concentration Mupirocin 25mg Sterile Distilled Water 5ml Directions: Warm up the refrigerated solution to room temperature and aseptically add 5 ml in 500 ml sterile, molten cooled (45-50°C) Bifidobacteria Selective Count Agar Base (BSC Propionate Agar Base) M1734 . Mix well and pour into sterile Petri plates. Storage and Shelf Life Store at 2-8°C. Use before expiry date on label.



100045 (1.00045.0010) (10支/盒)  100045 MUP Selective Supplement Supplement for the preparation of TOS-MUP Medium 
Typical Composition (per vial) Lithium mupirocin 25 mg
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食品微生物之檢驗方法-乳酸菌之檢驗(公告檢驗方法)
2.2.21.2. 轉半乳糖苷寡醣-莫匹羅星培養基(Transgalactosylated oligosaccharides-mupirocin medium, TOS-MUP) 基礎培養基(Basic medium):轉半乳糖苷寡醣-丙酸鹽 培養基(TOS-propionate agar medium) 蛋白腖(peptone) 10 g 酵母抽出物(yeast extract) 1 g  3 磷酸二氫鉀(KH2PO4) 3 g 磷酸氫二鉀(K2HPO4) 4.8 g 硫酸銨((NH4)2SO4) 3 g 硫酸鎂(MgSO4.7H2O) 0.2 g L-半胱氨酸鹽(L-cysteine.HCl.H2O) 0.5 g 丙酸鈉(sodium propionate) 15 g 轉半乳糖苷寡醣(transgalactosylated oligosaccharides, TOS) 10 g 洋菜(agar) 15 g 蒸餾水 950 mL 攪拌加熱溶解後,每瓶分裝 190 mL,以 115℃滅菌 15 分鐘,最終 pH 值為 6.3±0.2。配製好之基礎培養基置 於 2~4℃冷藏條件下,可儲存 1 週。 莫匹羅星補充溶液(MUP supplement solution) 稱取莫匹羅星鋰(lithium mupirocin) 50 mg 溶於蒸餾水 50 mL,以無菌膜過濾,臨用時配製。 完全培養基(Complete medium) 取配製好之基礎培養基,加熱溶解冷卻至 48±1℃,每 190 mL 加入莫匹羅星補充溶液 10 mL(最終濃度為 50 mg/L),小心混勻避免氣泡產生,冷卻至約 48℃使用。 
101 年 6 月 7 日署授食字第 1011902050 號公告 102 年 9 月 6 日部授食字第 1021950329 號公告修正
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